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rat igg2a antibody  (R&D Systems)


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    Structured Review

    R&D Systems rat igg2a antibody
    Rat Igg2a Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rat+igg/Rat+IgG2A+PE-conjugated+Antibody/pm42806145-1084-0-4
    Average 93 stars, based on 58 article reviews
    rat igg2a antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis
    Article Snippet: .. Allophycocyanin (APC)-conjugated anti-rat IgG (R&D Systems) was used to stain rat anti-FGFR2IIIb antibody (clone 133730; R&D Systems). .. Rat IgG2a (clone 2A3; Bio X Cell) was used as the isotype control.

    Article Title: Injectable Genetic Engineering Hydrogel for Promoting Spatial Tolerance of Transplanted Kidney in Situ
    Article Snippet: Subsequently, overnight at 4 °C, the tissue slices were incubated with antigen‐specific primary antibodies, including CD169 (1:300, Abcam), I‐Ad (1:300, Abcam), TCRβ (1:300, Abcam), GL7 (1:300, Abcam), B220 (1:300, Abcam), CD4 (1:300, Abcam), CD8 (1:300, Abcam), FOXP3 (1:300, Abcam), PDL1 (1:300, Abcam), CD31 (1:300, Abcam), CD41 (1:300, Abcam), CD68 (1:300, Abcam), IgG (1:300, Abcam), C3 (1:300, Abcam), collagen I (1:300, Abcam), collagen III (1:300, Abcam), and α‐SMA (1:300, Abcam). .. This was followed by incubation with secondary antibodies of Alexa Fluor 488 conjugated Goat Anti‐Rabbit IgG, Cy3 conjugated Donkey Anti‐Rabbit IgG, Alexa Fluor 488 conjugated Goat Anti‐Rat IgG and Cy5 conjugated Donkey Anti‐Rat IgG (R&D Systems), following with DAPI staining. .. The fluorescence images were acquired using confocal microscopy (Zeiss, LSM 900).

    Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis.
    Article Snippet: .. Allophycocyanin (APC)-conjugated anti-rat IgG (R&D Systems) was used to stain rat anti-FGFR2IIIb antibody (clone 133730; R&D Systems). .. Rat IgG2a (clone 2A3; Bio X Cell) was used as the isotype control.

    Incubation:

    Article Title: Injectable Genetic Engineering Hydrogel for Promoting Spatial Tolerance of Transplanted Kidney in Situ
    Article Snippet: Subsequently, overnight at 4 °C, the tissue slices were incubated with antigen‐specific primary antibodies, including CD169 (1:300, Abcam), I‐Ad (1:300, Abcam), TCRβ (1:300, Abcam), GL7 (1:300, Abcam), B220 (1:300, Abcam), CD4 (1:300, Abcam), CD8 (1:300, Abcam), FOXP3 (1:300, Abcam), PDL1 (1:300, Abcam), CD31 (1:300, Abcam), CD41 (1:300, Abcam), CD68 (1:300, Abcam), IgG (1:300, Abcam), C3 (1:300, Abcam), collagen I (1:300, Abcam), collagen III (1:300, Abcam), and α‐SMA (1:300, Abcam). .. This was followed by incubation with secondary antibodies of Alexa Fluor 488 conjugated Goat Anti‐Rabbit IgG, Cy3 conjugated Donkey Anti‐Rabbit IgG, Alexa Fluor 488 conjugated Goat Anti‐Rat IgG and Cy5 conjugated Donkey Anti‐Rat IgG (R&D Systems), following with DAPI staining. .. The fluorescence images were acquired using confocal microscopy (Zeiss, LSM 900).

    Article Title: Soluble E-cadherin Drives Brain Metastasis in Inflammatory Breast Cancer
    Article Snippet: .. SDS-PAGE and immunoblotting were done as previously described by us., Membranes were incubated with the corresponding primary antibodies overnight at 4°C and then incubated with secondary antibodies (1:5000) anti-rat IgG (#HAF005, R&D Systems) and anti-rabbit IgG (#7074, Cell Signaling) for 2 h at room temperature. ..

    SDS Page:

    Article Title: Soluble E-cadherin Drives Brain Metastasis in Inflammatory Breast Cancer
    Article Snippet: .. SDS-PAGE and immunoblotting were done as previously described by us., Membranes were incubated with the corresponding primary antibodies overnight at 4°C and then incubated with secondary antibodies (1:5000) anti-rat IgG (#HAF005, R&D Systems) and anti-rabbit IgG (#7074, Cell Signaling) for 2 h at room temperature. ..

    Western Blot:

    Article Title: Soluble E-cadherin Drives Brain Metastasis in Inflammatory Breast Cancer
    Article Snippet: .. SDS-PAGE and immunoblotting were done as previously described by us., Membranes were incubated with the corresponding primary antibodies overnight at 4°C and then incubated with secondary antibodies (1:5000) anti-rat IgG (#HAF005, R&D Systems) and anti-rabbit IgG (#7074, Cell Signaling) for 2 h at room temperature. ..

    Immunohistochemistry:

    Article Title: An immunohistochemical atlas of necroptotic pathway expression
    Article Snippet: Secondary antibodies for immunoblotting (1:10,000 working concentration) were horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulin (Ig) (Southern BioTech Cat#3010-05), HRP-conjugated goat anti-rabbit Ig (Southern BioTech Cat#4010-05), and HRP-conjugated goat anti-mouse Ig (Southern BioTech Cat#1010-05). .. Reagents for immunohistochemistry were HRP-conjugated anti-rabbit Ig (Agilent Cat# K400311-2), HRP-conjugated anti-mouse Ig (Agilent Cat# K400111-2), ImmPRESS HRP-conjugated anti-rat IgG for human samples (Vector Laboratories Cat#MP740450), HRP-conjugated anti-rat IgG for mouse samples (R&D Systems Cat#VC005-125), HRP-conjugated anti-digoxigenin antibody (Cell Signaling Technology, clone D8Q9J, Cat#49620), Rabbit Linker (Agilent Cat#GV80911-2). .. Epitope Retrieval Solution 1 (Leica Cat#AR9961), Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2), Retrieval Solution High pH (Agilent Cat#GV80411-2), 3,3’-diaminobenzidine (DAB) substrate (Agilent Cat#GV82511-2 or GV92511-2), Dako REAL Peroxidase-blocking reagent (Agilent Cat#S202386-2), bluing reagent (Leica, Cat#3802915), Background Sniper (Biocare Medical Cat#BS966L), Dako Protein Block (Agilent Cat#X0909), ‘Normal’ block (Agilent Cat#S202386-2), EnVision FLEX TRS High pH (Agilent Cat# GV80411-2), EnVision FLEX TRS Low pH (Agilent Cat# GV80511-2), MACH4 universal HRP polymer (Biocare Medical Cat#M4U534L), and DPX (Trajan Cat#EUKITT), Opal TSA-Dig (Akoya Cat#OP-001007) and Opal Polaris 780 reagent (Akoya Cat#OP-001008).



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    Image Search Results


    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biomedical Journal

    Article Title: Anti-PD-L1 improves barrier function and reduces CD103 + DC cell accumulation by regulating gut and lung microbiota and its metabolites to alleviate asthma in juvenile mice

    doi: 10.1016/j.bj.2025.100944

    Figure Lengend Snippet: The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Based on previous [ , , ] studies with appropriate modifications and our previous pre-experimental protocol, the anti-mouse PD-L1 monoclonal antibody (10 μg/g/mouse, BioXcell, 804722F1B, America) and rat IgG2b (Clone: LTF-2) isotype control antibody (10 μg/g/mouse, BioXcell, 767921D1, America) were administered by i.p. injection on days 0, 7, and 14 of sensitization and 21, 28, and 35 of challenge.

    Techniques: Expressing

    Antibodies in this study.

    Journal: Journal of Biochemical and Molecular Toxicology

    Article Title: Eupatilin Alleviates Acute Myocardial Ischemia‐Reperfusion Injury in Rats via the Akt/GSK‐3β Pathway by Targeting COXs

    doi: 10.1002/jbt.71111

    Figure Lengend Snippet: Antibodies in this study.

    Article Snippet: HRP‐conjugated Goat anti‐Rat IgG (H + L) , AS028 , WB: 1:7000 IHC: 1:1000 , Abclonal.

    Techniques: